Gel zur Aufarbeitung von komplexen Proteinproben

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Feldlinien im Quadrupol

If samples were applied to a gel for cleaning several aspects interfere:

Runtime long enough to separate contaminations
Runtime short enough to hold the proteins together
Runtime long enough to allow high-molecular proteins to run into the gel

Our recommendations:

- Separating gel with 20% PAA
- For monitoring, we recommend that you always include a "Prestained" marker.
- Always run the samples until the running front with the blue marker has clearly separated from the proteins.
- The ultimate runtime depends on the molecular weight of the largest protein of interest. (see recovery rate of a 120kDa protein in our experiments)
- Always stain the gel with Coomassie to be sure to cut out all proteins of interest.

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